Gateway clonase ii manual

the intermediate entry clone, refer to the Gateway® Technology with Clonase® II manual for a one-tube protocol. Although this protocol allows you to generate expression clones more rapidly than the standard BP reaction followed by the LR reaction, fewer expression clones will be obtained (generally 10–20% of the total number of entry clones).File Size: KB. gateway technology clonase ii manual hence simple! Besides being able to read most types of ebook files, you can also use this app to get free Kindle books from the Amazon store. Gateway Technology Clonase Ii Manual Gateway® Technology with Clonase® II A universal technology to clone DNA sequences for functional analysis and expression in. LR Clonase™ II enzyme mix is supplied as a 5X solution. If you wish to scale the reaction volume, make sure the LR Clonase™ II enzyme mix is at a final concentration of 1X. For a positive control, use ng (2 μL) of pENTR™-gus. 1. Add the following components to a mL microcentrifuge tube at room temperature and mix:File Size: KB.

For more information on the Gateway® Technology, refer to the Gateway® Technology with Clonase™ II manual. This manual is available for downloading from our Web site (www.doorway.ru) or by contacting Technical Support (page 20). attP Sequence Variations The attP sites between the pDONR™ vectors will contain. With the Gateway cloning system, a PCR fragment is first cloned into an Entry Vector using standard cloning techniqes (i.e. DNA ligase). The resulting clone may then be transferred to one or more functional Destination Vectors using the LR clonase enzyme. The LR clonase cloning step is very easy and efficient, requiring little time or effort. Gateway® BP Clonase® II enzyme mix catalyzes the in vitro recombination of PCR products or subcloning DNA segments from clones (containing attB sites) and a donor vector (containing attP sites) to generate entry www.doorway.ruy® BP Clonase® II contains enzymes and buffer in a single mix to enable convenient ten-microliter reaction set up with fewer pipetting steps.

Gateway Cloning Technology - Instruction Manual 1. GATEWAY™ Cloning Technology Note: This product is covered by Limited Label Licenses (see Section ). By use of this product, you accept the terms and conditions of the Limited Label Licenses. 2. and with BP Clonase II enzyme. • 90% of the colonies contain the Entry clone with the gene of interest in the correct orientation • Final Entry clones are ready for recombination with any Invitrogen™ Gateway™ Destination vector Learn which products to implement at each stage. LR Clonase™ II enzyme mix is supplied as a 5X solution. If you wish to scale the reaction volume, make sure the LR Clonase™ II enzyme mix is at a final concentration of 1X. For a positive control, use ng (2 μL) of pENTR™-gus. 1. Add the following components to a mL microcentrifuge tube at room temperature and mix.